Analyzing the Viability of Surface-Enhanced Raman Spectroscopy for the Accessible Detection of Toxic “Forever Chemicals” (PFAS) in Drinking Water

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Student: Andrew Glasgow
University:
UW-Madison
Major: Civil and Environmental Engineering, Environmental Emphasis
Graduation date: December 2023
Mentor: Haoran Wei

Summarize the research and your role.

The research was aimed at developing a novel, innovative approach for detecting per- and polyfluoroalkyl substances (PFAS) in drinking water. Despite PFAS exposure’s strong links to various cancers and other health effects, current PFAS detection approaches are very expensive and time-consuming, rendering them inaccessible to many communities — especially marginalized groups and those without financial resources. This inaccessibility is made even more pressing because marginalized communities are more likely to be continually exposed to high levels of PFAS in their drinking water. 

Together with my advisors, Hanwei Wang and Dr. Haoran Wei, I worked to assess the viability of surface-enhanced Raman spectroscopy (SERS) as a low-cost approach for the rapid detection of PFAS in drinking water. My role consisted of preparing, testing, and analyzing experimental data for different PFAS species, to determine if our experimental approach could help detect various compounds. I likewise had the unique opportunity to choose many of the next steps for experimental and variable analysis, and gained experience with advanced scientific instrumentation (e.g., scanning electron microscopy). Much of my work during the research program was accomplished in an independent manner, with my advisors being available for consultation as needed.

What skills have you gained?

The most valuable skill was gaining a strong comfort with the research process. Most of my prior research work had been “automatic,” with next steps being self-evident due to the nature of the experiments. My PFAS research through the SROP required a much more intensive and nuanced approach, as a slew of confounding variables (e.g., chemical properties) could potentially be influencing the results we obtained. Learning to trace and navigate the potential influence of these factors was an arduous process at first, but one that I believe made me a much more versatile researcher.

What was your favorite part of this project?

My favorite part was the opportunity to connect with like-minded individuals who are passionate about research that impacts the environment and society. Over the summer, one graduate student shared with me the importance of developing friendships with one’s coworkers, as they serve as a powerful motivator when the research process inevitably becomes discouraging. I found this piece of wisdom to be unequivocally true through my summer research experience and hope to continue to apply it during my future research endeavors.

What are your plans after graduate?

In autumn 2024, I will begin pursuing a Ph.D. in Environmental Engineering at Virginia Tech. In the interim between my graduation and the start of my doctoral studies, I have been working as a research assistant in two laboratories to gain additional experience with PFAS and contaminant research. Career-wise, I hope to become an environmental chemistry professor, to improve public health through the development of novel detection and treatment approaches for contaminants.

How will this experience help you attain your career goals?

This experience has provided me with significant knowledge and skills that will allow me to navigate graduate school and a career in the environmental chemistry field more easily. The experience solidified my dedication to scientific research that can improve society. For example, a true hope of mine one day is to aid in addressing the widespread and marked PFAS contamination in Okinawa, Japan. This goal stems directly from my research through the SROP, as the experience allowed me to fully realize my passion for research that can tangibly address severe environmental injustices.

Smart Farming and Precision Agriculture

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Aiden Drew and Josh Pederson created a video about their work. Watch the video.

Below is a Q&A with Aiden Drew.

Student: Aiden Drew
University: UW-Stout
Major: Applied Mathematics and Computer Science
Expected graduation date: May 2025
Mentors: Ahmed Elmagrous, Saleh Alneali, Keith Wojciechowski

Summarize the research and your role.

My research is on smart farming and precision agriculture. My role is to develop new ideas and build prototypes of new technologies that would be useful for growers. One of these technologies is a weather box that is placed in the grower’s field, which captures real-time data and sends it back to the grower by a mobile app.

What skills did you learn? 

I have learned important team-oriented skills like team communication and time effectiveness. I am able to be creative in the way I approach a problem. There are no wrong answers, just ideas. 

What are some of the opportunities you’ve had because of this research?

I’ve been able to attend different research conferences. At these conferences, I’m not only able share my research, but I also receive good questions/feedback from outside perspectives. 

What kind of career do you hope to go into after graduation?

I hope to go into data science/analysis, where I can use different mathematical methods to approach data visualization.

How will this experience help you attain your career goals?

I am able to work with large sums of data, where I can learn new ways to approach data analysis/visualization. Working with experts in the field, I am able to learn from the best in terms of how to tackle different scenarios with math.

Sex Based Spawning Behavior Differences in Lake Michigan Walleye

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Student: Carly Phalen
University: UW-Madison
Major: Wildlife Ecology with a certificate in Environmental Studies
Expected graduation date: December 2024
Mentor: Dan Isermann, UW-Stevens Point

Summarize the research and your role.

My role as an intern was to assist on a diverse array of graduate projects, including trout sampling in streams, conducting bass nest surveys, setting drift net arrays for sturgeon, and much more.

What skills have you gained?

Through this experience, I have acquired many skills, including electrofishing, eDNA sampling, otolith removal, drift and trawl net sampling, PIT tagging, and data analysis.

What was your favorite part of this project?

My favorite part of this experience was electrofishing for brook trout in spring ponds. These sampling sites were gorgeous, with crystal clear waters, and I enjoyed being hands on with such a beautiful fish species.

Students electrofishing

What are some of the opportunities you’ve had because of this research?

Through this experience, I was able to assist in an event at Kemp Station in which we gave kids a hands-on learning experience focused on engaging them in fisheries.

What are your plans after graduate?

After graduation, I hope to have a conservation-based career. I’m hoping to have a career conserving reptiles and amphibians.

This experience gave me many new skills necessary for a career in wildlife ecology, including a season conducting field research and data analysis. I believe these will be especially useful for my future, since I am planning on going to graduate school.

Steamer NIAGARA: Her Demise, Her Artifacts, and Her Significance

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Student: Nick Quaney
University: UW-Milwaukee
Major: History with a minor in English and a certificate in Celtic Studies
Expected graduation date: May 2025
Mentor: Kevin Cullen, Wisconsin Maritime Museum

Summarize the research and your role.

My research was at the Wisconsin Maritime Museum, going through their shipwreck artifact collection, specifically artifacts from the steamer NIAGARA, and researching maker’s marks. I was the only student researcher, working under the collections manager and curator.

What skills did you learn? 

I learned quite a bit about the many databases on maker’s marks, specifically British manufacture during the mid-19th century, the requirements, and procedures on how to handle artifacts, as well as the practical and less-flashy information on how museums operate, the structure of personnel and operations, and the making of museum exhibits.

What has been your favorite part about this experience?

Connecting with the museum staff and their partners at NOAA was one of my favorite parts, and the unique experience of looking at actual shipwreck artifacts.

What are some of the opportunities you’ve had because of this research?

I have had the opportunity to present my research at multiple symposia, in addition to the many connections I’ve made with academic advisors, curators, and NOAA federal employees.

What kind of career do you hope to go into after graduation? 

I hope to go to graduate school for a PhD in history, with a focus on military history, and to eventually teach at a university.

How will this experience help you attain your career goals?

I have made so many connections through my research, and the people I have met are more than willing to help me achieve my goals. The skills I gained have added to not just the way I look at my own field, but also the practical application of my studies, both of which are invaluable.

Coastal Bird Use of Small Stream Mouths Along the Western Lake Michigan Shoreline

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Student: Sarah Baughman
University: UW-Green Bay
Major: Biology with emphasis on Conversation and Ecology
Expected graduation date: May 2025
Mentor: Erin Giese

Summarize the research and your role.

My research focused on coastal bird use of small to medium-sized stream mouths along the western Lake Michigan shoreline. The findings of this project are intended to inform restoration projects and contribute to research on lesser known and understudied creeks and streams around the Great Lakes. I took part in all phases of this project, so I was able to gain experience with proposal development, volunteer outreach and coordinating, site scouting, surveying, data collection and analysis, report writing, and presentation of results. 

Female student conducting field work

What skills did you learn? 

As an undergraduate student, I’ve had the privilege of holding a variety of roles in this project and I learned something with each phase. I gained experience with writing a proposal, communicating with advisors and volunteers, and protocol development. I gained self-confidence as I navigated communicating the goals of this project to others and asking for access to private lands. My bird identification skills have increased, and I have felt capable of joining other bird-related research projects. In presenting at Research in the Rotunda, I was able to break through anxieties about public speaking, and I am now a more confident and thorough communicator. All of this to say: I have learned so much.

What has been your favorite part about this experience?

Seeing my personal evolution from the start of this project to the present and encouraging other students to embrace similar opportunities.

What are some of the opportunities you’ve had because of this research?

Bird researcher for the Great Lakes Coastal Wetland Monitoring Program; student research presenter at multiple symposia and conferences including the Chancellor’s Open House in 2023; Audubon Fly-In in Washington, DC; Cat Island Piping Plover Conservation Team Member.

Female student conducting field research

What kind of career do you hope to go into after graduation? 

Wildlife biologist; volunteer outreach; researcher and field team leader

How will this experience help you attain your career goals?

This experience has strengthened my confidence and communication skills; a foundation I can build future career choices on. I am a more competitive undergraduate student than I would have been without this experience.

Economic Impacts of Wisconsin Fishing Supported by The Freshwater Resources of Lake Michigan and Bay of Green Bay

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience analyzing how different fishery management strategies and climate change scenarios may affect the quality, and therefore economic value, of Lake Michigan and the Bay of Green Bay. Changes in quality and economic value in turn affect the health of regional economies and welfare of residents and visitors. Ultimately, this information helps inform resource managers about the most efficient and effective strategies available to maximize the value of this resource now and in the future.

University: UW-Whitewater
Mentor: Matthew Winden
Students:
Alaina Jacobs
Major: Entrepreneurship
Expected graduation date: May 2026

Clare O’Donnell
Major: Environmental Science
Expected graduation date: May 2024

Summarize the research and your role.

Jacobs: This research consisted of estimating the economic value of Lake Michigan and the Bay of Green Bay. We conducted multiple surveys for anglers to gain more information on their expenditures.

O’Donnell: I was involved in writing sections of the literature review and referencing other surveys sent out about commercial/ recreational/ charter boat fisheries. I then created the section of the survey that collects data about commercial fisheries (this is ongoing) and helped review the survey in its entirety. I also helped customize and create the poster that we presented at Research in the Rotunda.

What skills did you learn? 

Jacobs: I learned how to use Qualtrics and how to create a literature review on Excel. 

O’Donnell: I learned how to reference past studies to aid in future research practices. I also learned how to properly write a literature review. I learned how to create surveys in a way that optimizes results while still being easy to navigate for respondents.

What has been your favorite part about this experience?

Jacobs: My favorite part of this experience has been working with the team. Our varying backgrounds and perspectives propelled our research process. 

O’Donnell: My favorite part of this experience has been collecting the data and learning how important having the numbers can be when it comes to future decision-making. I also enjoyed referencing similar studies and seeing what we did differently and how we can improve.

What are some of the opportunities you’ve had because of this research?

Jacobs: I have had the opportunity to connect with local legislators and professors. This research has put me into important spaces. I had the opportunity to work alongside my peers, who also have a passion for the intersection of science and economics. 

O’Donnell: This research has given me more relevant experience for the jobs I am interested in pursuing. It has allowed me to gain more experience in collaborative working and experience in how to properly conduct research.

What kind of career do you hope to go into after graduation? 

Jacobs: I want to go into environmental entrepreneurship. 

O’Donnell: I hope to work in something related to environmental policy, environmental social governance, environmental health and safety or environmental consulting.

How will this experience help you attain your career goals?

Jacobs: This experience has enhanced my ability to convey information effectively. I learned how to use Qualtrics and how to create a literature review in Excel. This high-impact practice strengthened my critical thinking skills, and that is a quality I will need as a business owner. 

O’Donnell: This experience helped me gain relevant experience that can be applicable to many of the jobs I am interested in. I have learned so much through this process, and I am ready to use all the skills I have learned in the workforce. 

Adsorption of PFAS in Nanoporous Solids

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Student: Olivia Stellpflug
University: UW-Stevens Point
Major: Chemical Engineering with a Waste Resources Minor and Environmental Ethics Certificate
Expected graduation date: May 2025
Mentor: Joe Mondloch

Summarize the research and your role.

I have now been in two different research labs on campus–both studying PFAS adsorption to test methods for removing it from water. My role in both labs has been to prepare samples and record data to gather results on how these methods are performing.

What skills did you learn? 

It has improved my lab skills greatly outside the classroom, including preparing reagents, measuring out samples, and collecting data. Part of this role is also learning about the chemistry we are utilizing and how to interpret results and move forward. The biggest skill I have gained experience with is problem solving and troubleshooting when samples are not behaving the way we expected, and we must alter our approach.

What has been your favorite part about this experience?

My favorite part of this experience is learning from mentors on campus and to be part of a potential solution to an environmental concern. 

What are some of the opportunities you’ve had because of this research?

This research has helped me have background experience to draw on for future roles. I have been able to participate in research presentations and talk to administrators and officials, and it’s helping me secure future positions.

What are your plans after graduation?

My number one priority is environmental issues, and my goal is to work toward solutions to clean our planet. I hope to find a career in engineering solutions and sustainability to protect nature.

How will this experience help you attain your career goals?

My research experience has helped me take the first step toward this goal by developing my lab skills, problem solving, and communication that will allow me to be successful in my career. It has also expanded the range of knowledge I have of environmental issues.

Overstimulation of the Innate Response by the Neonicotinoid Pesticide Thiamethoxam: How Does This Contribute to Observed Toxicity in Fish?

The 2024 Research in the Rotunda featured 16 Freshwater Collaborative-funded research projects. Students shared more about their experience.

Student: Nicole Kooij
University: UW-La Crosse
Major: Biology with a Biomedical Science concentration
Expected graduation date: May 2024
Mentor: Tisha King-Heiden

Summarize the research and your role.

I am the lead of my research, both carrying out the experimental process and data analysis with the help of my research mentor.  I started my research studying the effect of chronic exposure to a neonicotinoid pesticide, thiamethoxam, on the innate immune response of zebrafish. I carried out this experiment by measuring the amount of reactive oxygen species present in the organism at the selected time frame. This was then used to analyze whether the organism experienced a change in immune system functions.

What skills have you gained?

I gained skills such as reading and synthesizing primary literature and designing the experiment to test our hypothesis. Additionally, I used different statistical programs to analyze and interpret the data, which inspired me to examine the data.

What was your favorite part of this project?

My favorite part of this experience was being able to carry out my own experiment, and this has aided me in my graduate school applications.

What are your plans after graduate?

I will be attending graduate school for a Ph.D. in Microbiology and Immunology. 

Marissa Jablonski, Executive Director

Dr. Marissa Jablonski is an accomplished water engineer, environmental advisor, and plastics-reduction expert who has worked in more than 45 countries on Earth. Her way of understanding and engaging in complex interactions between human beings and environmental systems, combined with her skills in storytelling and systems processes, make her a much sought after consultant and public speaker.

Marissa lives her life as part of a global community. She is a founding member and mentor to the University of Wisconsin-Milwaukee student chapter of Engineers Without Borders, and advises on the design and implementation of projects in Guatemala. She also serves as International Coordinator of a Guatemalan-led NGO that builds infrastructure with indigenous communities’ to meet the needs of five groups involved in each project.

After earning a PhD in Environmental Engineering and serving as an instructor of Peacebuilding, Engineering, and Physics at numerous institutions in Milwaukee, Wisconsin she moved to Washington, DC to work in policy. During her time as a 2017-19 AAAS Science Technology and Policy Fellow at USAID, she was both a Public Diplomacy Fellow in the Office of American Schools and Hospitals Abroad and also an Environmental Advisor to the Office of Food For Peace. During this time, she was also asked by the US Embassy of Thailand and the Phuket Hotels Association to serve as an Embassy Science Fellow to design and lead a 5-point model to help reduce their single-use plastics by more than 6 million in 2018.

Marissa is an advocate for minorities and women in STEM fields and served as coordinator of NSF’s FORTE program during 2009-2015. During that time, she also designed an internationally recognized project that engaged with informal dye industries in rural India to affordably clean their wastewater. Marissa’s innovative outlook on research, business, and life has won her many awards and praise from groups that include the National Science Foundation, Philanthropic Education Organization, Mondialogo, and the Karnataka State Pollution Control Board.

Be sure to follow Marissa on InstaGram and Facebook @MarissaJablonski.

Freshwater Collaborative Funded Projects 2023-2025

The Freshwater Collaborative of Wisconsin awarded more than $4.34 million in funding for the FY24 and FY25 budget years. The 22 funded projects will increase research and training opportunities for high school and undergraduate students and will address Wisconsin’s biggest water challenges, including emerging contaminants such as PFAS and agricultural water management issues such as phosphorus pollution.

A Collaborative Research on Synthesis of Graphene Oxide (GO) from Sustainable Resources and Its Application for Removal of Per- and Polyfluoroalkyl Substances (PFAS) from Water
Institutions: UW-Stevens Point, UW-Madison
Principal Investigator(s): Seyed Javad Amirfakhri, UW-Stevens Point; Xuejeun Pan, UW-Madison

PFAS is a group of chemicals detected in the drinking water of millions of Americans due to their widespread applications. They have been linked to several health concerns. The main objective of this work is to synthesize graphene oxide (GO) from sustainable resources, such as walnut shells, and to investigate the performance of GO for PFAS removal from water. Several students from UW-Stevens Point and UW-Madison will be trained to perform the research. Moreover, they will participate in disseminating the results, collaborating with industrial partners, engaging our community with STEM education, and increasing public scientific literacy on PFAS contamination.

Continuation and Expansion of the Red Cedar Watershed Monitoring Project
Institutions: UW-Stout, UW-Eau Claire, UW Oshkosh, UW-River Falls: $11,400
Principal Investigator(s): Keith Gilland, Nicole Hayes, Julia Chapman, Amanda Little (UW-Stout)
Collaborators: Sarah Vitale, UW-Eau Claire; Jill Coleman-Wasik, UW-River Falls; Greg Kleinheinz, UW Oshkosh; Chase Cummings, Dunn County Land and Water Conservation District)

The Red Cedar Watershed experiences frequent blue-green algae blooms due to phosphorus pollution. Numerous projects have been implemented to reduce runoff and restore stream channels and buffer areas. This project continues to examine the effectiveness of those projects while expanding research efforts to include whole-ecosystem and watershed processes to determine the root causes of the toxic algal blooms regularly seen in lakes in the region. Students from UW-Stout, UW-River Falls, UW-Eau Claire, and UW Oshkosh will work during the summer to survey streams, riparian corridors, and wetlands while also monitoring Lakes Tainter and Menomin to help guide management decisions regarding the Red Cedar Watershed to serve as a model for other similarly impacted watersheds throughout the region.

Continuing the Work of the Data Analysis and Monitoring Crew
Institutions: UW-River Falls
Principal Investigator(s): Jill Coleman Wasik

The Data Analysis and Monitoring Crew (or DAM Crew) is a two-week, hands-on, field-based training experience for UW students who are interested in river health and restoration. Participants work directly with practicing environmental professionals to attain the skills to implement a monitoring plan to assess ecological and geomorphological changes in a riverway that result from dam removal. The DAM Crew is a public-private partnership among UW-River Falls, the City of River Falls, Interfluve Inc., the Kinni Corridor Collaborative, and Trout Unlimited. Participants gain in-demand technical skills, increase their professional network, and serve the River Falls community.

The Cost of Cleanwater: An Efficiency Analysis of Wisconsin’s Water Utilities
Institutions: UW-Whitewater
Principal Investigator(s): Russell Kashian

Students will conduct research through the Institute for Water Business on the efficiency of water utilities and will leverage previous research to identify how costs incurred by water districts in cleaning water leads to inefficiency and increased prices. This research seeks to identify the cost of remediation to provide regulators the information necessary to make informed determinations regarding contaminant assessments. Results will be available in a public report and submitted for peer reviewed publication.

Data Collection and Parameter Estimation for a Dry Bean Yield Response to Irrigation Model
Institutions: UW-Stout
Principal Investigator(s): Keith Wojciechowski

The goal of this project is to help growers manage water resources and potentially increase yield. The research team at UW-Stout will construct automated weather stations and place them in fields containing crops. These stations will collect a variety of weather-related and plant-related data. UW-Stout’s team will analyze this data to help inform the agronomy team at corporate partner, Chippewa Valley Bean, so they can better advise their growers. Students working on this project will help construct weather stations and analyze data. Conducting this research will help these students develop a nascent expertise in precision agriculture.

Deposition and removal of emerging contaminants in the Milwaukee Estuary Area of Concern
Institutions: UW-Madison, UW-Milwaukee
Principal Investigator(s): Inna Popova, UW-Madison; Laodong Guo, UWM

The Greater Milwaukee Estuary faces pollution from emerging contaminants, such as PFAS and pharmaceuticals, posing risks to both the environment and public health. These contaminants are removed from the water through natural processes and accumulate in sediment, where they can persist for long periods, threatening organisms and humans who come in contact with them. The pollution history of these contaminants in the estuary remains poorly understood. This collaborative research project involves the analysis of sediment cores to study the contaminants’ history and behavior. The findings will aid in managing and remediating aquatic contaminations.

Development of a Collaborative Undergraduate Research Experience to Improve Per- and Polyfluoroalkyl (PFAS) Adsorption in Nanoporous Solids
Institutions: UW-Stevens Point, UW-Milwaukee
Principal Investigator(s): Joseph Mondloch, UW-Stevens Point; Yin Wang, Shangping Xu, UWM

Per- and Polyfluoroalkyl substances (aka PFAS) are emerging contaminants in Wisconsin’s waterways. This project provides funding to develop a collaborative undergraduate research experience between UW-Stevens Point and UW-Milwaukee to develop new PFAS adsorption technology. Researchers will test our technology against PFAS contaminated waters including real-world samples from Wisconsin’s waterways. Hands-on experience using start-of-the-art instrumentation will prepare students to enter the workforce with experience in PFAS chemistry, analysis, and treatment. 

Engaging Undergraduate Students in Cutting-Edge Research on the Use of Earth Materials for the Removal of Contaminants including Per- and Polyfluorinated Substances (PFAS)
Institutions: UW-Parkside, UW-Milwaukee
Principal Investigator(s): Zhaohui Li, Lori Allen, UW-Parkside; Shangping Xu, Yin Wang, UWM

Polyfluoroalkyl substances (PFAS) are forever chemicals, indicating that they stay in water and the environment permanently. UW-Parkside and UW-Milwaukee will engage 10 undergraduate students per year to conduct cutting-edge research for PFAS and color dyes removal from water. In addition, they will conduct PFAS analyses using state-of-the-art instruments for water samples collected from local drainage and Lake Michigan. The results will help southeastern Wisconsin to develop strategies to remove emerging contaminants from water and to help protect the region from contamination by forever chemicals.

Environmental Science Fair at UWEC: Water, Water, Everywhere!
Institutions: UW-Eau Claire
Principal Investigator(s): Sarah Vitale

This new project is a one-day multi-disciplinary Environmental Science Fair at the UW-Eau Claire campus for up to 100 regional high school students and accompanying high school teachers/advisers. The fair will include hands-on breakout sessions, a panel, a plenary speaker, and a traditional program fair for participants to interact with environmental science faculty at UW-Eau Claire. Freshwater Collaborative programming will be advertised to participants.

Freshwater@UW: An Immersive Undergraduate Summer Research Opportunities Program for the University of Wisconsin System
Institutions: UW-Madison
Principal Investigator(s): Alison Mikulyuk
Collaborators: All UW campuses

The Freshwater@UW Summer Research Opportunities Program provides immersive, hands-on mentored research experiences to 27 promising undergraduates within the 13 member institutions of the Freshwater Collaborative. The program’s central aim is to support the growth of our freshwater research enterprise and freshwater workforce through collaborative, cross-system programming designed to train, recruit, retain and diversify the next generation of freshwater professionals. Funds will support the third and fourth year of implementation and continued program development as we strive to create new, high-impact opportunities for talented students to build their skill and cultivate relationships within the UW System to that will help them seek further training in freshwater science.

Freshwater Science across the Curriculum: Linked Outreach and Advanced Educational Activities in Western Wisconsin
Institutions: UW-Eau Claire, UW Oshkosh, UW-River Falls, UW-Stout
Principal Investigator(s): Sarah Vitale, UW-Eau Claire
Collaborators: Greg Kleinheinz, UW Oshkosh; Jill Coleman-Wasik, UW-River Falls; Keith Gilland, UW-Stout

This ongoing project includes two freshwater science field courses in western Wisconsin: one targeting junior and senior high school students and the other an advanced course designed for upper-level college students. These field-intensive, hands-on learning experiences introduce participants to a wide range of freshwater science topics with specialists from UW-Eau Claire, UW-Stout, UW-River Falls, and UW Oshkosh. Courses are open to students enrolled in high schools across Wisconsin or from any UW System campus respectively.

From Field to Laboratory: Hands-on Techniques for Students in Water Sciences
Institutions: UW-River Falls, UW-Madison
Principal Investigator(s): Bahareh Hassanpour, UW-River Falls; Grace Bulltail; UW-Madison

This course focuses on hands-on laboratory and field techniques for studying freshwaters. Researchers will provide training in practical aspects of field measurements and laboratory practices pre-and post-sampling for students. They will conduct field campaigns for various purposes; will discuss the complexity and uncertainty of fieldwork; and focus on obtaining and preserving samples, and appropriate labeling. The laboratory training will range from day-to-day tasks, such as properly pipetting, keeping detailed records, sample preparation, and storage, to more complex analytic work, such as analyzing water samples using analytical instruments. Faculty will also work with students work on data analysis and poster presentation.

Lead, facilitate, and support policy research for the UW Water Policy Network
Institutions: UW-Milwaukee
Principal Investigator(s): Melissa Scanlan
Collaborators: All UW campuses

The Center for Water Policy leads, facilitates and supports the UW Water Policy Network, which serves as a hub for government agencies, private sector, NGOs, media and other stakeholders to identify water policy collaborators and experts. This project will foster collaboration on water policy research and curriculum across UW System by strengthening relationships among multidisciplinary faculty, researchers and students working on freshwater policy. The center convenes the UW Water Policy Network for presentations and discussions around key policy issues identified in the Freshwater Collaborative of Wisconsin’s 10 Grand Water Challenges.

Mitigating PFAS Contamination of Groundwater: Biochar Sequestration of PFAS in Biosolid Leachate at the Field Scale
Institutions: UW-Green Bay, UW-MilwaukeePrincipal Investigator(s): Kpoti Gunn, Michael Holly, UW-Green Bay; Yin Wang, UWM

Sewage sludge or biosolids generated in Wisconsin are largely applied to agricultural lands. Through this practice, biosolids may be the most diffuse source PFAS contamination of groundwater resources. This project aims to evaluate onsite the PFAS immobilization performance of activated biochar incorporated in soils receiving biosolids, and to develop methods for PFAS analysis of soil and groundwater leachate. Four undergraduate students involved in the project will contribute to experimental setup, soil and water sampling; laboratory and data analysis; and results publication. The project will provide students and faculty with research experience critical to the development of an emerging contaminant workforce.

My River Adventures (MRA) Pre-College Camp
Institutions: UW-La Crosse
Principal Investigator(s): Monica Yang

The UW-La Crosse MRA camp is a six-day residential camp for incoming 7-12 grade students. Students will use the UW-La Crosse campus as their homebase while they enjoy a week of instructional sessions and visits to rivers in the Driftless region for hands-on fieldwork and lab activities in collaboration with UW- La Crosse faculty, local educators and community members. This camp fosters recruitment, access and aspirations for a career in STEM, specifically water-related sectors. Students will create connections with community leaders and educators who can introduce them to career paths and increase their interest in STEM post-high school.

Partnering to Boost Aquaculture Workforce Development in Wisconsin
Institutions: UW-Madison, UW-Milwaukee, UW-Stevens Point
Principal Investigator(s): Sharon Moen, UW-Madison; Dong-Fang Deng, UWM; Emma Hauser, UW-Stevens Point

“Where do we find young people interested in producing fish for food?” This is one of the most pressing concerns that Wisconsin food-fish farmers expressed in a recent needs assessment conducted by Wisconsin Sea Grant. To address this food security and workforce issue, collaborators from the University of Wisconsin campuses of Madison, Stevens Point and Milwaukee are cooperating with commercial fish farms and high schools, colleges, and universities with existing aquaculture programs to expand training opportunities for students across the state. The opportunities range from farm experiences and skill-building workshops to support for teams engaging in an annual aquaculture competition.

Pilot Project: Development of an In Vivo Method to Assess the Innate Immune Response in Fathead minnow Larvae
Institutions:UW-La Crosse, UW-Madison
Principal Investigator(s): Tisha King-Heiden, UW-La Crosse; Gavin Dehnert, UW-Madison

Two undergraduate students will work with faculty from UW-La Crosse and UW-Madison to develop a new bioassay to study the immune response of wild fish. As part of their training, they will job shadow at the Wisconsin State Lab of Hygiene to gain a deeper understanding of how bioassays are used in the field of environmental toxicology. They will meet with experts from the Wisconsin Department of Health to see how data from these bioassays can be used to inform water quality standards. Finally, they will network at science conferences to learn about job opportunities in the field of environmental toxicology.

Quantifying the Impact of Spatial and Temporal Variation in Hyporheic Zone Fluxes on Phosphorus Transport and Release in Wisconsin Streams and Rivers
Institutions: UW-Green Bay, UW-Madison
Principal Investigator(s): Erin Berns-Herrboldt, UW-Green Bay; Christopher Zahasky, UW-Madison

Riverbed sediments can be an important source of phosphorus to Wisconsin waterways, driving eutrophication and negatively impacting aquatic health, human health, and local economies. There is limited understanding of how groundwater–surface water exchange impacts river sediment phosphorus storage, and this study aims to quantify these processes. Students will characterize phosphorus and subsurface hydrology in stream sediments at two sites in central Wisconsin and conduct batch and column experiments on sediment samples to evaluate which biogeochemical conditions promote storage and release of phosphorus. Project findings are anticipated to inform land, nutrient, and water management decisions.

Training K12 Educators in Groundwater Science
Institutions: UW-Eau Claire
Principal Investigator(s): Sarah Vitale

This project entails a one-day groundwater workshop with K-12 educators in the Eau Claire Area School District, and a hands-on classroom experience for each participating educator with UW-Eau Claire faculty and undergraduate students. The workshop will include exploration of groundwater characteristics using physical flow models, field experience on the UW-Eau Claire campus well field, and a tour of the Eau Claire Municipal Water Treatment Plant. Participants will receive a groundwater model to keep and use in their classroom. The follow-up classroom experience will provide an opportunity for educators to see groundwater models used with their students and build connections to support other Freshwater Collaborative programming.

UW-Green Bay Pre-College Student Experiences in Freshwater, 2023-2025
Institutions: UW-Green Bay
Principal Investigator(s): Emily Tyner

UW-Green Bay Pre-College Student Experiences in Freshwater is the continuation and expansion of a project that will enhance community-based experiential learning opportunities for pre-college students and teachers around the Green Bay and Lake Michigan watersheds. The project will build a community of freshwater-focused educators and middle and high school students, link to statewide water experts, and engage a diversity of urban to rural communities within the UW-Green Bay region. Intended outcomes include building skills and career-oriented experiences for high school scholars interested in the water sector, recruiting students to UW-Green Bay and UW System water-centric programs, and expanding efforts toward equity, inclusion, and diversity of participants.

UW Oshkosh Comprehensive Freshwater Collaborative of Wisconsin Training, Community Engagement, Business Enterprise, Research, and Recruitment Program
Institutions: UW Oshkosh, UW-Eau Claire, UW-Stout
Principal Investigator(s): Greg Kleinheinz, UW Oshkosh
Collaborators: Sarah Vitale, UW-Eau Claire; Amanda Little, UW-Stout

UW Oshkosh will offer student training opportunities at the Environmental Research and Innovation Center (ERIC), a state-certified laboratory for many water testing parameters as well as a contract R&D laboratory for various community and industry projects. The partnership with the Freshwater Collaborative allows students from any UW campus opportunities to work at ERIC field research sites (or take a field course) each summer, which embeds students in communities to study surface, well and groundwater. Funding from the Freshwater Collaborative will also allow for an on-campus STEM high school camp in summer 2024 (20 high school students). It will also provide resources for faculty-student research and student-industry projects. Freshwater Collaborative funding will continue to expand opportunities through the UW Oshkosh Freshwater 101 course (BIO/ENG 119) and partial support for a summer field sampling and analysis course open to all UW students. Finally, UW Oshkosh will continue to offer access to a research and teaching boat on the Lake Winnebago system at no charge to Freshwater Collaborative partners.

Water, Health, and Habitat Interactions: Building Capacity for Water Careers and Education
Institutions: UW-Milwaukee, UW-Green Bay,  UW-La Crosse, UW-Parkside, UW-River Falls, UW-Whitewater
Principal Investigator(s): Tracy Boyer
Collaborators: Chris Houghton, UW-Green Bay; Tisha King-Heiden, UW-La Crosse; Julie Kinzelman, UW-Parkside; Kevin Thaisen, UW-River Falls; Elisabeth Harrahy, UW-Whitewater

UW-Milwaukee will lead a collaboration with five UW campuses to implement three intensive hands-on courses that were developed specifically for the Freshwater Collaborative. These summer courses will provide undergraduate students throughout UW System with an affordable opportunity to conduct research and field work on Lake Michigan. These courses also create a nucleus of classes for future planned freshwater certificate offerings.

This project will also expand a UWM field course, based on feedback from industry partners, to make it more accessible to students on other campuses or those working full time. Faculty will also build an intensive series of specialized aquaculture courses that complement workforce development efforts. In addition, UWM will host a daylong field work experience aboard the R/V Neeskay for undergraduates participating in the Freshwater@UW Summer Scholars Program, a statewide Freshwater Collaborative initiative led by UW-Madison that places undergraduates in research programs throughout UW System.

Collaborative Course Offerings Include:

  • Environmental and Health Effects of Water Pollution. This is the hands-on component of a two-part course taught jointly by faculty from UWM, UW-La Crosse and UW-Whitewater.
  • Expedition to Lake Michigan. This hands-on course, taught by UWM and UW-River Falls faculty, focuses on the hydrology and biogeochemistry of Lake Michigan and incorporates a problem-based approaches to solve real problems affecting Lake Michigan.
  • Human Interactions with Lake Michigan Coastal Ecosystems. This four-week course led by UWM, UW-Green Bay and UW-Parkside explores the coast of Lake Michigan and will facilitate a greater understanding of human impacts on its coastal ecosystems.